Analysis Note

ControlSK-BR-3 cells

Application

Research CategoryEpigenetics & Nuclear FunctionCell Signaling

Flow Cytometry Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in HER-2 expressing SK-BR-3 cells (Montgomery, R. B., et al. (2005). Cancer Res. 65(2):650-656.).

Western Blot Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in HER-2 expressing SK-BR-3 cells (Montgomery, R. B., et al. (2005). Cancer Res. 65(2):650-656.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory precipitated c-ErbB2/c-Neu in IP (McKenzie, S. J., et al. (1989). 4(5):543-548.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected c-ErbB2/c-Neu in IF (McKenzie, S. J., et al. (1989). 4(5):543-548.).

Research Sub CategoryCell Cycle, DNA Replication & Repair

This Anti-c-ErbB2/c-Neu (Ab-5) Antibody, clone TA-1 is validated for use in Western Blotting, Flow Cytometry, Immunoprecipitation, Immunofluorescence for the detection of c-ErbB2/c-Neu (Ab-5).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The ErbB2 (HER2, c-Neu, ErbB2, c-erbB2, or HER2/neu) protein is member of the human epidermal growth factor receptor (HER) family. It is a receptor tyrosine kinase containing an intracellular kinase domain and an extracellular ligand domain, although no endogenous ligand has yet been found. ErbB2 dimerizes with ErbB1, 3, and 4, and can activate different pathways including the PI3K, PLCγ, and MAPK pathways, depending on the ErbB receptor involved. Coupling with ErbB3 activates the PI3K pathway, whereas coupling with any of these three receptors can induce the MAPK pathway. ErbB2 signaling promotes cell proliferation and inhibits apoptosis. ErbB2 is overexpressed in various cancers and may play a role in the development and metastasis of gliomas, and ovarian, breast, lung, and gastric cancer.

Immunogen

Epitope: Extracellular domain

Cells overexpressing human c-ErbB2/c-Neu

Linkage

Replaces: 04-1127

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ cultured supernatent in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Flow Cytometry in SK-BR-3 cells.

Flow Cytometry Analysis: 0.5 µg of this antibody detected c-ErbB2/c-Neu in 0.5X10E6 SK-BR-3 cells.

Specificity

This antibody recognizes the extracellular domain of ErbB2/c-Neu.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

138 kDa calculated